Partial Characterization of Lectin from Artocatpus sps

Plants are the most convenient and richest source of lectins due to their ease in isolation and availability. The present study was aimed to characterize the plant lectin isolated from the Artocarpus sps. After extraction of lectin from the jackfruit was subjected to SDS-PAGE which shows diverse biological active properties and also possible to examine their biological activity levels to elute and separate the bands of subunits of lectin from gel. In present study, it was found that all most all subunits are active and retains their natural biological activity of lectin. The present study revealed that smaller subunits of lectins has more agglutinating activity, it may be due to the more concentrated amount of the lectins subunits as the whole lectin. Native PAGE for the dialysed sample of Artocarpus sp. with different concentrations (25μl, 35μl, 45μl and 55μl) was shown that Purified lectin sample of Artocarpus sp. corresponds to 72 kDa with standard protein marker. According to the results of the present study lectin units are trimeric and have similar in lectin activity.